Plasmid

Part:BBa_K4404010

Designed by: Julia Fricke   Group: iGEM22_Goettingen   (2022-10-09)


acsB2 from C. ljungdahlii under the control of a PbgaL promoter from C. perfringens

This composite part consists of plasmid backbone pMTL83151 (BBa_K4404007) and acsB2 (BBa_K4404001) which is under the control of the PbgaL promoter (BBa_K2992023). The promoter from C. perfringens functions in Gram-negative (E.coli) and in Gram-positive (Clostridium saccharoperbutylacetonicum) bacteria and is inducible by lactose. The insert acsB2 codes for the acetyl-CoA synthase subunit (ACS) of the CODH/ACS-complex which catalyzes the formation of acetyl-CoA from CO and [CH3]-Co-FeS-P. acsB was inserted into the backbone pMTL83151 to complement the Wood-Ljungdahl metabolic pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2623
    Illegal EcoRI site found at 6098
    Illegal XbaI site found at 6125
    Illegal SpeI site found at 1777
    Illegal PstI site found at 542
    Illegal PstI site found at 5988
    Illegal PstI site found at 6164
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2623
    Illegal EcoRI site found at 6098
    Illegal NheI site found at 2230
    Illegal NheI site found at 6343
    Illegal SpeI site found at 1777
    Illegal PstI site found at 542
    Illegal PstI site found at 5988
    Illegal PstI site found at 6164
    Illegal NotI site found at 6067
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2623
    Illegal EcoRI site found at 6098
    Illegal BglII site found at 6152
    Illegal BamHI site found at 2214
    Illegal BamHI site found at 6119
    Illegal XhoI site found at 97
    Illegal XhoI site found at 6156
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2623
    Illegal EcoRI site found at 6098
    Illegal XbaI site found at 6125
    Illegal SpeI site found at 1777
    Illegal PstI site found at 542
    Illegal PstI site found at 5988
    Illegal PstI site found at 6164
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2623
    Illegal EcoRI site found at 6098
    Illegal XbaI site found at 6125
    Illegal SpeI site found at 1777
    Illegal PstI site found at 542
    Illegal PstI site found at 5988
    Illegal PstI site found at 6164
    Illegal NgoMIV site found at 3537
    Illegal NgoMIV site found at 5444
    Illegal NgoMIV site found at 5454
    Illegal NgoMIV site found at 5580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5196
    Illegal SapI.rc site found at 5551
    Illegal SapI.rc site found at 5922


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